Part:BBa_K2082009

Monobody: Example binding protein

Positive control for binding proteins from the Monobody Library BBa_K2082005.
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 166
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

This device consists of:

an Anderson promoter (BBa_J23106)

a RBS (BBa_B0034)

a rpoZ CDS (encoding RNA polymerase omega subunit)

a cMyc linker

the first constant region of a Monobody CDS

a specific randomized Monobody variable region

the second constant Region of a Monobody CDS

It is a single example Monobody fusion (BBa_K2082009) which is the perfect positive control for your designed Monobody library or other specific binders for colony PCRs, sequencings and more.

To get further insight into the theoretical assembly take a look here.
If you are planning to build your own Monobody library or construct a specific binder, take a look at our hints

This device is a component of our part collection, which also includes our designed Monobody library (BBa_K2082005) and devices(with or without rpoZ fusion) where you can exchange RFP CDS with regions for building your own library.

Visit our wiki for more information about our library and the opportunity of building your own library and further about our part collection. With this collection we provide every iGEM team the unprecedented chance for easily building their own Monobodies!

If you are interested in other binding proteins, check out our Nanobody parts for setting up a library.